SSR-based identification of genetic groups within European populations of Tuber aestivum Vittad.
Identifieur interne : 001002 ( Main/Exploration ); précédent : 001001; suivant : 001003SSR-based identification of genetic groups within European populations of Tuber aestivum Vittad.
Auteurs : Virginie Molinier [Suisse, France] ; Claude Murat [France] ; Martina Peter [Suisse] ; Armelle Gollotte [France] ; Herminia De La Varga [France] ; Barbara Meier [Suisse] ; Simon Egli [Suisse] ; Beatrice Belfiori [Italie] ; Francesco Paolocci [Italie] ; Daniel Wipf [France]Source :
- Mycorrhiza [ 1432-1890 ] ; 2016.
Descripteurs français
- KwdFr :
- Ascomycota (classification), Ascomycota (génétique), Ascomycota (isolement et purification), Europe (MeSH), Mycorhizes (classification), Mycorhizes (génétique), Mycorhizes (isolement et purification), Séquences répétées d'acides nucléiques (MeSH), Techniques de génotypage (MeSH), Techniques de typage mycologique (MeSH), Variation génétique (MeSH).
- MESH :
- génétique : Ascomycota, Mycorhizes.
- isolement et purification : Ascomycota, Mycorhizes.
- classification : Ascomycota, Europe, Mycorhizes, Séquences répétées d'acides nucléiques, Techniques de génotypage, Techniques de typage mycologique, Variation génétique.
English descriptors
- KwdEn :
- Ascomycota (classification), Ascomycota (genetics), Ascomycota (isolation & purification), Europe (MeSH), Genetic Variation (MeSH), Genotyping Techniques (MeSH), Mycological Typing Techniques (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (isolation & purification), Repetitive Sequences, Nucleic Acid (MeSH).
- MESH :
- classification : Ascomycota, Mycorrhizae.
- genetics : Ascomycota, Mycorrhizae.
- isolation & purification : Ascomycota, Mycorrhizae.
- Europe, Genetic Variation, Genotyping Techniques, Mycological Typing Techniques, Repetitive Sequences, Nucleic Acid.
Abstract
Tuber species are ectomycorrhizal ascomycetes establishing relationships with different host trees and forming hypogeous fruiting bodies known as truffles. Among Tuber species, Tuber aestivum Vittad. has a wide distributional range being found naturally all over Europe. Here, we performed large-scale population genetic analyses in T. aestivum to (i) investigate its genetic diversity at the European scale, (ii) characterize its genetic structure and test for the presence of ecotypes and (iii) shed light into its demographic history. To reach these goals, 230 ascocarps from different populations were genotyped using 15 polymorphic simple sequence repeat markers. We identified 181 multilocus genotypes and four genetic groups which did not show a clear geographical separation; although, one of them was present exclusively in Southeast France, Italy and Spain. Fixation index values between pairs of genetic groups were generally high and ranged from 0.29 to 0.45. A significant deficit of heterozygosity indicated a population expansion instead of a recent population bottleneck, suggesting that T. aestivum is not endangered in Europe, not even in Mediterranean regions. Our study based on a large-scale population genetic analysis suggests that genetically distinct populations and likely ecotypes within T. aestivum are present. In turn, this study paves the way to future investigations aimed at addressing the biological and/or ecological factors that have concurred in shaping the population genetic structure of this species. Present results should also have implications for the truffle market since defining genetic markers are now possible at least for some specific T. aestivum genetic groups.
DOI: 10.1007/s00572-015-0649-0
PubMed: 26070448
Affiliations:
- France, Italie, Suisse
- Bourgogne, Bourgogne-Franche-Comté, Grand Est, Lorraine (région)
- Bretenière, Champenoux, Dijon, Vandœuvre-lès-Nancy
- Université de Bourgogne, Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
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Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon, France. molinier.virginie@gmail.com.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR Agroécologie INRA, Agrosup, u. Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon</wicri:regionArea><placeName><region type="region">Bourgogne-Franche-Comté</region><region type="old region">Bourgogne</region><settlement type="city">Dijon</settlement></placeName><orgName type="university">Université de Bourgogne</orgName></affiliation></author><author><name sortKey="Murat, Claude" sort="Murat, Claude" uniqKey="Murat C" first="Claude" last="Murat">Claude Murat</name><affiliation wicri:level="4"><nlm:affiliation>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation></author><author><name sortKey="Peter, Martina" sort="Peter, Martina" uniqKey="Peter M" first="Martina" last="Peter">Martina Peter</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf</wicri:regionArea><wicri:noRegion>Birmensdorf</wicri:noRegion></affiliation></author><author><name sortKey="Gollotte, Armelle" sort="Gollotte, Armelle" uniqKey="Gollotte A" first="Armelle" last="Gollotte">Armelle Gollotte</name><affiliation wicri:level="3"><nlm:affiliation>Inoplant, Agronov, RD31, 21110, Bretenière, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inoplant, Agronov, RD31, 21110, Bretenière</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Bourgogne</region><settlement type="city">Bretenière</settlement></placeName></affiliation></author><author><name sortKey="De La Varga, Herminia" sort="De La Varga, Herminia" uniqKey="De La Varga H" first="Herminia" last="De La Varga">Herminia De La Varga</name><affiliation wicri:level="4"><nlm:affiliation>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName></affiliation></author><author><name sortKey="Meier, Barbara" sort="Meier, Barbara" uniqKey="Meier B" first="Barbara" last="Meier">Barbara Meier</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf</wicri:regionArea><wicri:noRegion>Birmensdorf</wicri:noRegion></affiliation></author><author><name sortKey="Egli, Simon" sort="Egli, Simon" uniqKey="Egli S" first="Simon" last="Egli">Simon Egli</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf</wicri:regionArea><wicri:noRegion>Birmensdorf</wicri:noRegion></affiliation></author><author><name sortKey="Belfiori, Beatrice" sort="Belfiori, Beatrice" uniqKey="Belfiori B" first="Beatrice" last="Belfiori">Beatrice Belfiori</name><affiliation wicri:level="1"><nlm:affiliation>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia</wicri:regionArea><wicri:noRegion>Perugia</wicri:noRegion></affiliation></author><author><name sortKey="Paolocci, Francesco" sort="Paolocci, Francesco" uniqKey="Paolocci F" first="Francesco" last="Paolocci">Francesco Paolocci</name><affiliation wicri:level="1"><nlm:affiliation>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia</wicri:regionArea><wicri:noRegion>Perugia</wicri:noRegion></affiliation></author><author><name sortKey="Wipf, Daniel" sort="Wipf, Daniel" uniqKey="Wipf D" first="Daniel" last="Wipf">Daniel Wipf</name><affiliation wicri:level="4"><nlm:affiliation>UMR Agroécologie INRA, Agrosup, u. Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR Agroécologie INRA, Agrosup, u. Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon</wicri:regionArea><placeName><region type="region">Bourgogne-Franche-Comté</region><region type="old region">Bourgogne</region><settlement type="city">Dijon</settlement></placeName><orgName type="university">Université de Bourgogne</orgName></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (classification)</term><term>Ascomycota (genetics)</term><term>Ascomycota (isolation & purification)</term><term>Europe (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Genotyping Techniques (MeSH)</term><term>Mycological Typing Techniques (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (isolation & purification)</term><term>Repetitive Sequences, Nucleic Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (classification)</term><term>Ascomycota (génétique)</term><term>Ascomycota (isolement et purification)</term><term>Europe (MeSH)</term><term>Mycorhizes (classification)</term><term>Mycorhizes (génétique)</term><term>Mycorhizes (isolement et purification)</term><term>Séquences répétées d'acides nucléiques (MeSH)</term><term>Techniques de génotypage (MeSH)</term><term>Techniques de typage mycologique (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Ascomycota</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Europe</term><term>Genetic Variation</term><term>Genotyping Techniques</term><term>Mycological Typing Techniques</term><term>Repetitive Sequences, Nucleic Acid</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Ascomycota</term><term>Europe</term><term>Mycorhizes</term><term>Séquences répétées d'acides nucléiques</term><term>Techniques de génotypage</term><term>Techniques de typage mycologique</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tuber species are ectomycorrhizal ascomycetes establishing relationships with different host trees and forming hypogeous fruiting bodies known as truffles. Among Tuber species, Tuber aestivum Vittad. has a wide distributional range being found naturally all over Europe. Here, we performed large-scale population genetic analyses in T. aestivum to (i) investigate its genetic diversity at the European scale, (ii) characterize its genetic structure and test for the presence of ecotypes and (iii) shed light into its demographic history. To reach these goals, 230 ascocarps from different populations were genotyped using 15 polymorphic simple sequence repeat markers. We identified 181 multilocus genotypes and four genetic groups which did not show a clear geographical separation; although, one of them was present exclusively in Southeast France, Italy and Spain. Fixation index values between pairs of genetic groups were generally high and ranged from 0.29 to 0.45. A significant deficit of heterozygosity indicated a population expansion instead of a recent population bottleneck, suggesting that T. aestivum is not endangered in Europe, not even in Mediterranean regions. Our study based on a large-scale population genetic analysis suggests that genetically distinct populations and likely ecotypes within T. aestivum are present. In turn, this study paves the way to future investigations aimed at addressing the biological and/or ecological factors that have concurred in shaping the population genetic structure of this species. Present results should also have implications for the truffle market since defining genetic markers are now possible at least for some specific T. aestivum genetic groups. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26070448</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>SSR-based identification of genetic groups within European populations of Tuber aestivum Vittad.</ArticleTitle><Pagination><MedlinePgn>99-110</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-015-0649-0</ELocationID><Abstract><AbstractText>Tuber species are ectomycorrhizal ascomycetes establishing relationships with different host trees and forming hypogeous fruiting bodies known as truffles. Among Tuber species, Tuber aestivum Vittad. has a wide distributional range being found naturally all over Europe. Here, we performed large-scale population genetic analyses in T. aestivum to (i) investigate its genetic diversity at the European scale, (ii) characterize its genetic structure and test for the presence of ecotypes and (iii) shed light into its demographic history. To reach these goals, 230 ascocarps from different populations were genotyped using 15 polymorphic simple sequence repeat markers. We identified 181 multilocus genotypes and four genetic groups which did not show a clear geographical separation; although, one of them was present exclusively in Southeast France, Italy and Spain. Fixation index values between pairs of genetic groups were generally high and ranged from 0.29 to 0.45. A significant deficit of heterozygosity indicated a population expansion instead of a recent population bottleneck, suggesting that T. aestivum is not endangered in Europe, not even in Mediterranean regions. Our study based on a large-scale population genetic analysis suggests that genetically distinct populations and likely ecotypes within T. aestivum are present. In turn, this study paves the way to future investigations aimed at addressing the biological and/or ecological factors that have concurred in shaping the population genetic structure of this species. Present results should also have implications for the truffle market since defining genetic markers are now possible at least for some specific T. aestivum genetic groups. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Molinier</LastName><ForeName>Virginie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland. molinier.virginie@gmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR Agroécologie INRA, Agrosup, u. Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon, France. molinier.virginie@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murat</LastName><ForeName>Claude</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peter</LastName><ForeName>Martina</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gollotte</LastName><ForeName>Armelle</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Inoplant, Agronov, RD31, 21110, Bretenière, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De la Varga</LastName><ForeName>Herminia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>UMR1136 Interactions Arbres-Microorganismes, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>INRA, UMR1136 Interactions Arbres-Microorganismes, F-54280, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meier</LastName><ForeName>Barbara</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Egli</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Swiss Federal Institute for Forest Snow and Landscape Research (WSL), 8903, Birmensdorf, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Belfiori</LastName><ForeName>Beatrice</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paolocci</LastName><ForeName>Francesco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>National Research Council, Institute of Biosciences and BioResources-Perugia (CNR-IBBR), 06128, Perugia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wipf</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>UMR Agroécologie INRA, Agrosup, u. Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, Université de Bourgogne, 21065 Cedex, Dijon, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005060" MajorTopicYN="N">Europe</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060005" MajorTopicYN="Y">Genotyping Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016533" MajorTopicYN="Y">Mycological Typing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012091" MajorTopicYN="N">Repetitive Sequences, Nucleic Acid</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Europe</Keyword><Keyword MajorTopicYN="N">Microsatellites markers</Keyword><Keyword MajorTopicYN="N">Population genetics</Keyword><Keyword MajorTopicYN="N">Tuber aestivum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>05</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26070448</ArticleId><ArticleId IdType="doi">10.1007/s00572-015-0649-0</ArticleId><ArticleId IdType="pii">10.1007/s00572-015-0649-0</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>PLoS One. 2013 Dec 16;8(12):e82353</Citation><ArticleIdList><ArticleId IdType="pubmed">24358175</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2011 Jun;48(6):592-601</Citation><ArticleIdList><ArticleId IdType="pubmed">20965267</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2012 May;21(9):2116-29</Citation><ArticleIdList><ArticleId IdType="pubmed">22429247</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2014 Apr;24 Suppl 1:S101-13</Citation><ArticleIdList><ArticleId IdType="pubmed">24604084</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 1998 Aug;7(8):963-74</Citation><ArticleIdList><ArticleId IdType="pubmed">9711862</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2012 May;194(3):614-28</Citation><ArticleIdList><ArticleId IdType="pubmed">22489902</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2008;180(2):466-78</Citation><ArticleIdList><ArticleId IdType="pubmed">18643942</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2015 Jan;25(1):77-81</Citation><ArticleIdList><ArticleId IdType="pubmed">24989673</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3166-70</Citation><ArticleIdList><ArticleId IdType="pubmed">8159720</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Biol. 2011 Jul;115(7):569-97</Citation><ArticleIdList><ArticleId IdType="pubmed">21724164</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2010 Nov;19(22):4994-5008</Citation><ArticleIdList><ArticleId IdType="pubmed">21040049</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2010 Dec;19(23):5216-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21044190</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2012 Oct 1;28(19):2537-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22820204</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2006;170(1):177-84</Citation><ArticleIdList><ArticleId IdType="pubmed">16539614</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2005 Jul;170(3):1261-80</Citation><ArticleIdList><ArticleId IdType="pubmed">15520263</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2002 Oct;4(10):584-94</Citation><ArticleIdList><ArticleId IdType="pubmed">12366753</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Jul;199(1):176-87</Citation><ArticleIdList><ArticleId IdType="pubmed">23574460</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2010 Oct;30(10):1311-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20688880</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2006 Apr;72(4):2390-3</Citation><ArticleIdList><ArticleId IdType="pubmed">16597935</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2005 Nov;71(11):7279-84</Citation><ArticleIdList><ArticleId IdType="pubmed">16269769</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1973 Dec;70(12):3321-3</Citation><ArticleIdList><ArticleId IdType="pubmed">4519626</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2014 Apr;24 Suppl 1:S89-100</Citation><ArticleIdList><ArticleId IdType="pubmed">24272144</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2015 Aug;17 (8):3039-50</Citation><ArticleIdList><ArticleId IdType="pubmed">26036799</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Plant Sci. 2013 Jan 31;1(2):null</Citation><ArticleIdList><ArticleId IdType="pubmed">25202513</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2014 Apr;24 Suppl 1:S115-25</Citation><ArticleIdList><ArticleId IdType="pubmed">24563212</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1999 Dec;153(4):1989-2000</Citation><ArticleIdList><ArticleId IdType="pubmed">10581301</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol Resour. 2015 Sep;15(5):1243-55</Citation><ArticleIdList><ArticleId IdType="pubmed">25703414</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2007 May;16(9):1811-22</Citation><ArticleIdList><ArticleId IdType="pubmed">17444894</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hered. 1998 May-Jun;89(3):238-47</Citation><ArticleIdList><ArticleId IdType="pubmed">9656466</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2004 Jun 1;235(1):109-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15158269</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013;8(1):e52765</Citation><ArticleIdList><ArticleId IdType="pubmed">23300990</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1996 Dec;144(4):2001-14</Citation><ArticleIdList><ArticleId IdType="pubmed">8978083</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2008 Oct 1;24(19):2222-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18710873</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2011 Feb;21(2):131-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20499111</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>Italie</li><li>Suisse</li></country><region><li>Bourgogne</li><li>Bourgogne-Franche-Comté</li><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Bretenière</li><li>Champenoux</li><li>Dijon</li><li>Vandœuvre-lès-Nancy</li></settlement><orgName><li>Université de Bourgogne</li><li>Université de Lorraine</li></orgName></list><tree><country name="Suisse"><noRegion><name sortKey="Molinier, Virginie" sort="Molinier, Virginie" uniqKey="Molinier V" first="Virginie" last="Molinier">Virginie Molinier</name></noRegion><name sortKey="Egli, Simon" sort="Egli, Simon" uniqKey="Egli S" first="Simon" last="Egli">Simon Egli</name><name sortKey="Meier, Barbara" sort="Meier, Barbara" uniqKey="Meier B" first="Barbara" last="Meier">Barbara Meier</name><name sortKey="Peter, Martina" sort="Peter, Martina" uniqKey="Peter M" first="Martina" last="Peter">Martina Peter</name></country><country name="France"><region name="Bourgogne-Franche-Comté"><name sortKey="Molinier, Virginie" sort="Molinier, Virginie" uniqKey="Molinier V" first="Virginie" last="Molinier">Virginie Molinier</name></region><name sortKey="De La Varga, Herminia" sort="De La Varga, Herminia" uniqKey="De La Varga H" first="Herminia" last="De La Varga">Herminia De La Varga</name><name sortKey="De La Varga, Herminia" sort="De La Varga, Herminia" uniqKey="De La Varga H" first="Herminia" last="De La Varga">Herminia De La Varga</name><name sortKey="Gollotte, Armelle" sort="Gollotte, Armelle" uniqKey="Gollotte A" first="Armelle" last="Gollotte">Armelle Gollotte</name><name sortKey="Murat, Claude" sort="Murat, Claude" uniqKey="Murat C" first="Claude" last="Murat">Claude Murat</name><name sortKey="Murat, Claude" sort="Murat, Claude" uniqKey="Murat C" first="Claude" last="Murat">Claude Murat</name><name sortKey="Wipf, Daniel" sort="Wipf, Daniel" uniqKey="Wipf D" first="Daniel" last="Wipf">Daniel Wipf</name></country><country name="Italie"><noRegion><name sortKey="Belfiori, Beatrice" sort="Belfiori, Beatrice" uniqKey="Belfiori B" first="Beatrice" last="Belfiori">Beatrice Belfiori</name></noRegion><name sortKey="Paolocci, Francesco" 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